Technical requirements for autoclaves - Huaqiang Electronic Network

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1.

(1) Add appropriate amount of water to the water stop line before using the pot. It is advisable to use hot water that has been opened to prevent the accumulation of scale and shorten the sterilization time.

(2) Put the items to be sterilized into the pot, not too tight, so as not to affect the circulation and sterilization effect of the steam. Do not place the item near the wall of the pot to prevent condensation from flowing into the item along the silver wall.

(3) Open the exhaust valve, heat it, and then close the exhaust valve after 3-5 minutes of steam generation. At this time, the steam has exhausted the cold air out of the pot. Let the temperature rise with the increase of steam pressure. . When the pressure gradually rises to the required pressure, the heat source is controlled to maintain the time required for sterilization. Generally controlled at 1kg/cm2, ie 1.034×10^5Pa, sterilized for 20min.

(4) When the sterilization is finished, turn off the heat source. When the pressure drops to 0, immediately open the lid and take out the items. Do not put them for a long time. Prevent water vapor from condensing on the lid and the four walls to form water droplets, falling onto the sterilized items, moistening the wrapping paper, and increasing the possibility of contamination of the fire-borne articles.

(5) The store can be used to take a small amount of medium and put it in a 37 ° C incubator for 24 hours. If it is sterile, it can be stored for later use. The slant culture medium is taken out from the pot and placed in a hot slope. After solidification, it is placed in a 37 ° C incubator for 24 hours. If it is aseptically grown, it can be stored for later use.

2. Technical requirements for the use of fully automatic high pressure steam sterilizer

(1) First check the water line that regulates the life of the tank, which should be at the high water level of the tank.

(2) Check the water level at the bottom of the sterilizer. The water level line should just have not passed the bottom; when the power is turned on, the display starts to flash and the water is turned on. Also confirm that the steam discharge valve is closed.

(3) Put the items to be sterilized into the sterilizer, push the beam to the left, the beams must all be pushed into the left column, turn the handle clockwise, so that the lid is pressed down the pot, and the force is allowed to make it Separate.

(4) Select the sterilization procedure, there are two ways of automatic autoclave sterilization procedure. That is, manual sterilization procedures and automatic sterilization procedures. The temperature and time of sterilization are set by the buttons on the operation panel.

(5) Press the start button to start working. At this time, the display will flash continuously. When the temperature inside the sterilizer reaches 80 °C or above, the display will start to display the actual temperature inside the bacteria. When the temperature exceeds 100 °C After that, the gauge pointer on the display begins to indicate the actual pressure within the sterilizer. When the set temperature is reached, the system automatically adjusts the pressure and temperature inside the vessel for a predetermined period of time.

(6) After the article is sterilized, it needs to be quickly dried. The safety valve must be opened or the exhaust valve should be turned to the left to rotate the car to the "open" position, so that the steam in the sterilizer is quickly discharged, so that the residual water vapor on the article is quickly evaporated. However, it is strictly prohibited to use a drying method when sterilizing liquids.

(7) After the sterilization is completed, after checking that there is no pressure in the chamber, the handle can be turned counterclockwise to open the door to take out the sterilized articles and send them into the sterilization item storage room.