in
Neural progenitor
(NPC) separation and amplification processes must be evaluated functionally by assessing their ability to differentiate into multiple neural lineages
Pluripotency
. The kits presented here provide a quick and easy technique to confirm the ability of your starting cell population to differentiate into astrocytes, neurons, and oligodendrocyte lineages.
The ability to verify pluripotency in the early stages of the experimental process:
√ Define the starting stem cell population √ Reduce variability in experiments and between studies √ Ensure efficient use of time and reagents
Summary of the Neurological System Function Identification Kit
• NPC is cultivated using media supplements formulated for short-term maintenance and expansion.
• Using neural differentiation additives, NPC can differentiate into astrocytes, neurons, and oligodendrocyte lineages.
• The pluripotency of NPc can be functionally verified using an antibody panel that detects phenotypic specific markers of neural progenitor cells, astrocytes, neurons, and oligodendrocytes.
• All media additives and marker antibodies are included in this kit.
Content of the Neurological System Function Identification Kit
• Neuron Maintenance Additives • Neuronal Differentiation Additives • Bovine Fibronectin 100X
• Goat anti-rat nestin antigen affinity purified polyclonal antibody • Sheep anti-human GFAP antigen affinity purified polyclonal antibody • Mouse anti-neuron specific b-III tubulin monoclonal antibody • Mouse anti-small gelatin Cytoplasmic marker O4 monoclonal antibody
Assay Principle
Typical Data
Verification of pluripotency of neural progenitor cells. Neural progenitor cells were maintained and differentiated into the neural lineage using a dedicated medium additive provided in the Human/Mouse/rat Neural lineage Functional Identification Kit (Catalog No. Sc028). The pluripotency of neural progenitor cells is functionally verified using an antibody assay plate (provided in the kit) that detects phenotypic specific markers of neural precursors, astrocytes, neurons, and oligodendrocytes.
Elisa kit, imported elisa kit, elisakit, imported serum, imported reagent, gelatin zymography test kit, rat elisa test kit, human elisa test kit-Nanjing Xinfan Biotechnology Co., Ltd.
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