Determination of alcohol in blood by headspace chromatography - Database & Sql Blog Articles

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Determination of alcohol content in blood by headspace chromatography

Beijing Huasheng Spectrum Instrument Co., Ltd.

When using this method to analyze the ethanol content in the blood of the perpetrator or victim in a traffic accident, you need to understand and pay attention to the following questions to facilitate accurate and reliable testing.

:

An internal standard (tebutanol) analysis method must be used. Compared with the general analysis method, it is more important to select the appropriate internal standard for headspace chromatography, and the impact on the analysis results is more accurate.

The blood alcohol content is usually called blood alcohol content, and the unit of blood alcohol content is expressed in three types:

meaning

unit

How many milligrams of ethanol per milliliter of blood

Mg/mL

each

100

How many milligrams of ethanol are in the milliliter of blood?

Mg%

each

100

How many grams of ethanol are in the ml of blood?

%

Usually the last two units are used in the identification report, for example, using a series of lectures (

3

Condition, the blood alcohol content of the driver in the traffic accident is measured per ml of blood

1.10

In milligrams of ethanol, the three units are expressed as:

11.10mg/mL2110mg%30.11%.

The measured value in the formal appraisal report is a set of accurate data with legal effects. It is used to judge whether the driver is an important basis for drunk driving or drunk driving. The physiological response is obviously different due to different blood alcohol levels.

Blood alcohol concentration and drunkenness

Blood alcohol concentration

Mg%

)

Drinking symptoms

<50

Almost asymptomatic

,

No difference from normal person status

,

However, there is a small difference in judgment and response.

50-100

Red, euphoric, floating

,

Accurate examination reveals eye sensitivity and coordination

Have a reduction

,

Those who do not have alcohol consumption experience may have an accident while driving.

100-150

More words, excitement, self-review

,

Lack of reason, easy to irritate

,

Dilated pupils, nystagmus, ambiguous speech, ataxia, facial congestion or blueness, and control of motor vehicles are prone to danger.

150-250

Uncoordinated action

,

Confusion of consciousness

,

Heavy tongue

,

Never drive

250-350

Walking difficulty

,

Ambiguous

,

Bright eyes

,

Start to enter a coma.

300-400

Drowsy state

,

Breathing

,

Decreased body temperature

,

No response to stimuli, life-threatening.

400-500,

Deep coma

,

Breathing paralysis and dying

.

The different concentrations of blood alcohol were obtained from the experimental average to classify the symptoms of alcohol into the above seven categories, which is the symptom development process of most drunken people. People have great individual differences in tolerance to alcohol, and some human blood alcohol concentrations are

30mg%

There is drunkenness, some people

150mg%

Still self-sustaining. The symptoms of fasting drinkers are serious and develop rapidly. The symptoms of eating and drinking are slow and light. In most cases, the blood alcohol concentration is

200-350mg%

Most of them will be drunk.

A large number of test data are collected to obtain the proportional relationship between each organ and blood alcohol. In order to facilitate the bloodless sample, the ethanol content of other samples is multiplied by the proportional coefficient, and the blood alcohol content can also be obtained indirectly. For the use of the appraisers. The scale factor is as follows: the amount of ethanol in the blood is

1.00

The average brain content is

1.17

The average urine content is

1.35

The average liver content is

0.91

The content in saliva is

1.30

The content of blood clots is

0.77

Plasma content is

1.16

Serum content is

1.12

Beijing Huasheng Spectrum Instrument Co., Ltd. manufactures and sells

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