Nanjing Xinfan Bio: What are the new ways to play ChIP?

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Biotech reports: If researchers want to know what proteins are doing, they usually take a direct approach: co-immunoprecipitation knows proteins

A

And protein

B

Are there together,

ChIP

Able to identify protein

A

Whether with nucleic acid sequences

B

Combine.

Chromatin immunoprecipitation

ChIP

Can identify binding to a given protein

DNA

The basic concept of the sequence can be traced back to decades. now

ChIP

It has become a basic tool for epigenomics, and it can be used in conjunction with next-generation sequencing to achieve localization and map analysis of genomic regulatory regions.

In the past, people tend to focus most of their energy on protein coding regions, Stanford University School of Medicine.

Michael Snyder

Said the professor. "It is very obvious that the regulatory area is also very important.

ChIP

It is almost the best way to find out these regulatory areas. ”

ChIP

The experiment is mainly to crosslink the protein

-DNA

The interaction is fixed, breaking the cells and releasing the chromatin. Will then

DNA

Break into small pieces and capture what we want with antibodies against the target protein

DNA

. Final removal of cross-linking recovery

DNA

And pass

PCR

,

DNA

Chip or sequencing for detection.

ChIP

The underlying philosophy hasn't changed much over the years, but developers have been continually optimizing. Let’s take a look at the occasion of resigning the old and welcoming the new

ChIP

There are new things in the field that are worth looking forward to. (Extended reading: success

ChIP

Three tips

)

Handling low volume cells

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Traditional

ChIP

It is often necessary to use a large number of cells, one million or more. For researchers who deal with clinical samples throughout the day,

FACS

Sorting cells and

FFPE

It is difficult for cells to meet such requirements.

under these circumstances,

Active Motif

company's

ChIP-IT® High Sensitivity kit

It is a good choice. From the product data, this kit is suitable for handling low-volume cells (

1000

Cells) or low abundance proteins (

50,000

Cells), especially some rare transcription factors. “This is one of our best-selling products,” said the company’s product manager.

Kyle Hondorp

Say.

Get the latest information on the ChIP-IT® High Sensitivity kit now

If you want to get it like this

DNA

Sequencing, you can try it

Kapa Biosystems

company's

KAPA Hyper Prep Kit

. This kit can be used

ChIP

Low amount generated

DNA

(

100 pg

) construct a sequencing library,

Kapa

Company support and application manager

Adriana Geldart

Introduce the road.

recent

Active Motif

The company also released low-volume cells

ChIP-Seq

Technical service, you only need to provide 10,000 cells, you can throw the relevant work to them.

Solid state

ChIP

Traditional

ChIP

Coupling protein

A

Or protein

G

Agarose or magnetic beads to precipitate antibodies

-

Chromatin complex.

Chromatrap

The company transformed this process into a new filter-based approach.

Chromatrap

Analytical protein

A

Or protein

G

The coated porous filter replaces the beads and saves a lot of

ChIP

Related traditional liquid handling steps. "It is basically a solid state

ChIP

analysis,"

Chromatrap

company's

Stephen Knight

Point out.

This approach can significantly reduce sample loss during the elution and pipetting steps. “After we load, the sample is left in the matrix,” Product Development Manager

Amy Beynon

Say, "The elution is only

30

Seconds, while the beads take five minutes. "Of course, this has been greatly shortened.

ChIP

The time required.

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